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An inexpensive microcomputer-based image-analysis system: novel applications to quantitative autoradiography.

A Isseroff·D Lancet
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Abstract

We describe a relatively inexpensive, yet versatile and powerful microcomputer-based image-analysis system, and its applications to processing of deoxyglucose autoradiographic data. Images are acquired via a video camera mounted on a light microscope or a light box, and digitized in 40 ms to 512 X 512 picture elements with 8-bit resolution (256 gray levels). The bit-mapped image analysis hardware can provide up to 256 colors for pseudo-color coding, and virtually instantaneous readout of brightness values for densitometry. The system is controlled by an 8-bit S-100 bus microcomputer, providing flexibility and ease of expansion. In addition to pseudo-color coding and densitometry, we have developed programs for averaging of successive sections, image subtraction and quantitative reconstruction of different planes of section from serial autoradiograms.

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AnimalsAutoradiographyBrainBrain MappingComputersGlucoseMicrocomputersNeurophysiologyRatsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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A Isseroff, D Lancet. 1985. An inexpensive microcomputer-based image-analysis system: novel applications to quantitative autoradiography.. https://doi.org/10.1016/0165-0270(85)90011-1

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