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Cell-cycle analysis in 20 minutes.

H A Crissman·R A Tobey
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Abstract

Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.

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AnimalsCell DivisionCell LineCells, CulturedCricetinaeCytological TechniquesDNADNA ReplicationFemaleFluorescenceFluorometryHeLa CellsHumansKineticsLungMethodsMitosisOvaryPlicamycinSpectrometry, FluorescenceStaining and LabelingJournal Article

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H A Crissman, R A Tobey. 1974-06-21. Cell-cycle analysis in 20 minutes.. https://doi.org/10.1126/science.184.4143.1297

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