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Microtiter solid-phase radioimmunoassay for hepatitis B antigen.

R H Purcell·D C Wong·H J Alter·P V Holland
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Abstract

A micro-solid-phase radioimmunoassay (micro-SPRIA) for hepatitis B antigen (HB Ag) was developed for use with microtiter serological equipment. Radiolabeled immunoglobulin G was prepared from human and animal sera containing hepatitis B antibody (HB Ab); it was not necessary to isolate specific HB Ab by immunochemical means. A micro-SPRIA prepared with guinea pig reagents was approximately as sensitive as the AusRIA radioimmunoassay, but, like the AusRIA test, yielded false positive results. A micro-SPRIA prepared with human reagents was slightly less sensitive but did not yield false positive results. These micro-SPRIA tests offer several advantages, including conservation of reagents, adaptability to other antigen-antibody systems, ease of performance (especially when testing large numbers of specimens), and economy.

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AnimalsAntibody SpecificityDrug StabilityGuinea PigsHaplorhiniHepatitisHepatitis B AntigensHumansImmune SeraImmunoglobulin GIodine IsotopesMacacaMethodsRadioimmunoassaySilverJournal Article

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R H Purcell, D C Wong, H J Alter, P V Holland. 1973. Microtiter solid-phase radioimmunoassay for hepatitis B antigen.. https://doi.org/10.1128/am.26.4.478-484.1973

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