Renin inactivation during in vitro experiments.

K W Cho; R L Malvin

doi:10.1152/ajprenal.1979.236.5.f501

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Renin inactivation during in vitro experiments.

K W Cho·R L Malvin

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Abstract

Experiments were designed to clarify the factors affecting renin released during in vitro experiments. Kidneys from rat, dog, and pig were used. Experiments were done in which the gas phase was either bubbled through the incubation medium or layered above it. Renin released into the incubation medium disappeared very rapidly when gas was bubbled through the medium. The decline was similar in mediums bubbled with oxygen-CO2 (95%--5%) or nitrogen-CO2 (95%--5%). The half-life of renin activity in the bubbled medium was approximately 15 min in both cases. However, in experiments in which nonbubbled medium was used throughout, renin released into the incubation medium did not disappear after removal of slices. These data are interpreted to mean that the renin released into the incubation medium is inactivated at the air-water interface.

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Animals Carbon Dioxide Dogs Half-Life In Vitro Techniques Kidney Oxygen Rats Renin Species Specificity Swine Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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K W Cho, R L Malvin. 1979. Renin inactivation during in vitro experiments.. https://doi.org/10.1152/ajprenal.1979.236.5.f501

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