Intracellular immunoglobulins. A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates.

J Burns; M Hambridge; C R Taylor

doi:10.1136/jcp.27.7.548

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Intracellular immunoglobulins. A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates.

J Burns·M Hambridge·C R Taylor

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Abstract

Formalin fixation followed by routine paraffin embedding allows the demonstration of intracellular immunoglobulin by a sandwich technique using either peroxidase or fluorescein isothyocyanate labelled antibody conjugates. The results compared favourably with those obtained using either the fresh frozen or the Sainte-Marie method for preserving tissue immunoglobulins. The formalin-paraffin method with peroxidase-labelled antibody has advantages for routine use. Morphology is excellent, preparations are permanent, and retrospective studies of stored paraffin-embedded tissue are possible. Some of the problems of labelled antibody studies are discussed.

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Animals Fluoresceins Fluorescent Antibody Technique Formaldehyde Histological Techniques Hodgkin Disease Humans Immune Sera Immunoglobulin M Immunoglobulins Lymph Nodes Paraffin Peroxidases Rabbits Spleen Swine Thiocyanates Comparative Study Journal Article

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J Burns, M Hambridge, C R Taylor. 1974. Intracellular immunoglobulins. A comparative study on three standard tissue processing methods using horseradish peroxidase and fluorochrome conjugates.. https://doi.org/10.1136/jcp.27.7.548

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