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PubMed · 4610176

New method for large-scale preparation of covalently closed lambda DNA molecules.

R Reuben·M Gefter·L Enquist·A Skalka
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Abstract

A combination of mutations in bacteriophage lambda and its host Escherichia coli K-12 provides a convenient system for the isolation of large quantities of covalently closed circular DNA molecules. We describe two procedures for the large scale preparation of lambda DNA in the duplex circular form.

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BacteriolysisCentrifugation, Density GradientColiphagesDNA ReplicationDNA VirusesDNA, CircularDNA, ViralEscherichia coliEthidiumLysogenyMutationPhosphorus RadioisotopesRecombination, GeneticThymineTritiumJournal Article

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R Reuben, M Gefter, L Enquist, A Skalka. 1974. New method for large-scale preparation of covalently closed lambda DNA molecules.. https://doi.org/10.1128/jvi.14.5.1104-1107.1974

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