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PubMed · 6114805

A flexible, computer-controlled video microscope capable of quantitative spatial, temporal, and spectral measurements.

E S Rich·J E Wampler
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Abstract

A video microscope system has been constructed and tested that incorporates computer-controlled video cameras for high-resolution and low-light microscopy. The low-light camera system involves a dual microchannel plate-image intensifier capable of photon gain as high as 500 000 and a gated silicone-intensified target vidicon to achieve usable photon sensitivity with a noise equivalent signal of only 2 photons (500 nm) per pixel per second. We have compared the limitations and capabilities of this camera system with those of a high-resolution video camera and conventional photomicroscopy. Uses of the low-light camera coupled to a computer system include image acquisition of weak-light images from self-luminous specimens, fluorescence microscopy with weak exciting light, kinetic resolution of calcium-mediated events as monitored by the calcium-sensitive bioluminescence of aequorin, and spatially resolved spectroscopic measurements. Flexible use of this system in these various applications is possible because it allows operation with illumination intensities over a dynamic range of 100 000:1.

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AnimalsCnidariaComputersEggsFishesLuminescent MeasurementsMicroscopyMicroscopy, FluorescenceOligochaetaPhotomicrographySpectrum AnalysisTelevisionJournal Article

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E S Rich, J E Wampler. 1981. A flexible, computer-controlled video microscope capable of quantitative spatial, temporal, and spectral measurements.. https://pubmed.ncbi.nlm.nih.gov/6114805/

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