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PubMed · 6167536

A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff.

K Yamada·H Kitamura·Y Maseki
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Abstract

A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines an alkaline phosphatase-labeled concanavalin A-5-bromo-3-indolyl phosphate, p-toluidine salt (Con A-ALP-BIPT) method with periodic acid-Schiff (PAS) sequence. With the present dual staining method, it is possible to color alpha-D-glucosyl and alpha-D-mannosyl residues blue and 1,2-glycol groups of neutral complex carbohydrates magenta. The validity of this method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.

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Alkaline PhosphataseAnimalsCarbohydratesCattleChickensConcanavalin ADogsHaplorhiniHistocytochemistryHumansMicroscopyPeriodic Acid-Schiff ReactionRatsStaining and LabelingSwineJournal Article

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K Yamada, H Kitamura, Y Maseki. 1981. A dual staining method for neutral complex carbohydrates using alkaline phosphatase-labeled concanavalin A and periodic acid-Schiff.. https://doi.org/10.1007/bf00508377

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