Identification of elastin in developing aortic tissue by immunological methods.

F W Keeley

doi:10.3109/03008208109152374

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Identification of elastin in developing aortic tissue by immunological methods.

F W Keeley

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Abstract

Antibodies raised in rabbits to tropoelastin isolated from lathyritic chick aorta were conjugated with ferritin and used to identify and locate elastin-containing elements in sections of aortic tissue from developing chicks. The ferritin-antibody conjugate was associated with a network of fine filaments with a diameter of 3-5 nm which, in the 8-10 day old chick embryo aorta, was distributed between and around small but recognizable elastic fibers. In older aortic tissue this ferritin-labelled material was seen principally in loose association with the periphery of developing elastic fibers. The microfibrils did not interact with the ferritin-antibody conjugate. Treatment of chicks with beta aminopropionitrile did not interfere with the accumulation of ferritin-labelled filaments on these small elastic fibers, suggesting that the mechanism of addition of new material onto growing fibers may be independent of cross-linking.

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Amino Acids Animals Antibodies Aorta Chick Embryo Chickens Elastin Immunodiffusion Molecular Weight Tropoelastin Journal Article Research Support, Non-U.S. Gov't

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F W Keeley. 1981. Identification of elastin in developing aortic tissue by immunological methods.. https://doi.org/10.3109/03008208109152374

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