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PubMed · 6668569

Horse lymphocyte cell synchronization: improved technique for chromosome analysis.

U Märki·D R Osterhoff
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Abstract

A method using methotrexate for horse lymphocyte cell synchronization and thymidine for incorporation into DNA replication is described. This method provides a powerful technique for the study of chromosomal abnormalities and detailed analysis of chromosomal replication patterns. The determination of horse karyotypes with many similar chromosomes needs a special method which reveals the numerous and informative stages of the cell cycle. Horse lymphocyte cell cultures treated with colcemid (20 min) and harvested 6 hours after the release of the 17 hour-block with methotrexate show the best results for exact identification and analysis of horse chromosomes.

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AnimalsCell CycleChromosome AberrationsChromosome BandingDNA ReplicationHorsesKaryotypingLymphocytesMethotrexateJournal Article

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U Märki, D R Osterhoff. 1983. Horse lymphocyte cell synchronization: improved technique for chromosome analysis.. https://pubmed.ncbi.nlm.nih.gov/6668569/

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