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Estrogen 2-hydroxylase: activity in rat tissues.

R L Barbieri·J A Canick·K J Ryan
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Abstract

Incubation parameters for a radioderivative assay for estrogen 2-hydroxylase have been examined. The assay was found to be specific and sensitive if a chromatographically purified preparation of COMT was used. Estradiol was found to be a better substrate for the 2-hydroxylase than estrone or estriol. The liver had significantly higher estrogen 2-hydroxylase activity than any other tissue examined. The estrogen 2-hydroxylase was highly localized in the microsomal fraction in both the liver and the brain. The male rat was found to have significantly more estrogen 2-hydroxylase activity in the liver than the female rat. In addition, in the male rat liver, the estrogen 2-hydroxylase activity was reversibly inducible by testosterone and was not affected by phenobarbital. In the male and female rat brain the estrogen 2-hydroxylase activities were similar.

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AnimalsBrainCastrationCatechol O-MethyltransferaseCytochrome P-450 CYP1A1EstrogensFemaleKidneyMaleMicrosomesMicrosomes, LiverOvaryPhenobarbitalRatsSteroid HydroxylasesSubcellular FractionsSubstrate SpecificityTestisTestosteroneComparative StudyJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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R L Barbieri, J A Canick, K J Ryan. 1978. Estrogen 2-hydroxylase: activity in rat tissues.. https://doi.org/10.1016/0039-128x(78)90064-8

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