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PubMed · 7363459

Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography.

M D Kolins·E Epstein·W H Civin·S Weiner
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Abstract

We describe a one-dimensional thin-layer chromatographic system for separation of amniotic fluid phosphatidylcholine, sphingomyelin, phosphatidylglycerol, phosphatidylinositol, phosphatidylserine, and phosphatidylethanolamine in amniotic fluid. We utilize short-bed continuous development and "high performance" thin-layer chromatography. Phospholipids are detected with an antimony molybdate staining reagent and quantitated by transmittance densitometry. This system is more sensitive to changes in lecithin/sphingomyelin ratios than are planimetric evaluations.

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Amniotic FluidChromatography, Thin LayerFemaleHumansMicrochemistryPhosphatidylcholinesPhosphatidylethanolaminesPhosphatidylglycerolsPhosphatidylinositolsPhosphatidylserinesPhospholipidsPregnancySphingomyelinsComparative StudyJournal Article

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M D Kolins, E Epstein, W H Civin, S Weiner. 1980. Amniotic fluid phospholipids measured by continuous-development thin-layer chromatography.. https://pubmed.ncbi.nlm.nih.gov/7363459/

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