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Quantitative analysis of quaternary ammonium antiseptics using thin-layer densitometry.

J Paesen·I Quintens·G Thoithi·E Roets·G Reybrouck·J Hoogmartens
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Abstract

A thin-layer chromatography method for quantitative analysis of quaternary ammonium antiseptics is described. Silanized silica gel was used as the stationary phase. The mobile phase consisted of methanol-25% (m/v) sodium acetate solution-acetone (65:35:20). The method is able to separate the chain homologues of benzalkonium chloride, cetylpyridinium chloride and cetrimide. Detection was performed using a colour reaction with potassium triiodide solution. The different homologues were quantified using UV densitometry at 400 nm. A number of commercial samples was analysed using this method. From the results it appears that it is worthwhile to have a limit test for the composition of quaternary ammonium antiseptics in pharmacopoeial monographs, the more so as the antibacterial activity depends on it.

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AcetatesAcetic AcidAcetoneAnti-Infective Agents, LocalBenzalkonium CompoundsCetrimoniumCetrimonium CompoundsCetylpyridiniumChromatography, Thin LayerMethanolQuaternary Ammonium CompoundsJournal Article

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J Paesen, I Quintens, G Thoithi, E Roets, G Reybrouck, J Hoogmartens. 1994-08-19. Quantitative analysis of quaternary ammonium antiseptics using thin-layer densitometry.. https://doi.org/10.1016/0021-9673(94)80165-7

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