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Human recombinant interferons-beta and -gamma decrease gelatinase production and invasion by human KG-2 renal-carcinoma cells.

K Gohji·I J Fidler·R Tsan·R Radinsky·A C von Eschenbach·T Tsuruo·M Nakajima
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Abstract

We investigated the effects of human recombinant interferons (r-IFNs) on gelatinase production and invasion by human renal-cell carcinoma (HRCC). Incubation of KG-2 HRCC with human r-IFN-beta or -gamma (but not -alpha) suppressed transcription of the 72-kDa gelatinase gene and, hence, production of gelatinase activity. These inhibitory effects of interferons (IFNs) were independent of their antiproliferative effects. Treatment of KG-2 cell with r-IFN-beta or -gamma significantly inhibited cell invasion through reconstituted basement membrane toward chemoattractants produced by kidney fibroblasts. The inhibitory activity of r-IFNs was specific to the KG-2 cells since gelatinase activity by various fibroblasts was unaffected. These findings suggest that r-IFN-beta or IFN-gamma may be used to inhibit the invasive potential of HRCC.

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Carcinoma, Renal CellCollagenFibroblastsGelatinasesHumansInterferon Type IInterferon beta-1aInterferon beta-1bInterferon-betaInterferon-gammaKidneyKidney NeoplasmsNeoplasm InvasivenessRNA, MessengerRecombinant ProteinsSkinTumor Cells, CulturedJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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K Gohji, I J Fidler, R Tsan, R Radinsky, A C von Eschenbach, T Tsuruo, M Nakajima. 1994-08-01. Human recombinant interferons-beta and -gamma decrease gelatinase production and invasion by human KG-2 renal-carcinoma cells.. https://doi.org/10.1002/ijc.2910580313

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