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PubMed · 8558718

Crystal-cell interaction inhibition by polysaccharides.

C F Verkoelen·J C Romijn·L C Cao·E R Boevé·W C De Bruijn·F H Schröder
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Abstract

PURPOSE: We studied the effect of polysaccharides on interactions between calcium oxalate monohydrate (COM) crystals and cultured renal cells. MATERIALS AND METHODS: Monolayers of Madin-Darby canine kidney (MDCK) cells were incubated with radiolabeled crystals in the presence of various concentrations of natural glycosaminoglycans (GAGs) and semisynthetic polysaccharides (SSPs). RESULTS: While most GAGs were found to have relatively little effect, SSPs (SP54, G871 and G872) were potent inhibitors of crystal-cell association. Pretreatment of crystals, but not of cells, was similarly effective, suggesting polysaccharide-induced modification of crystal surface properties. CONCLUSIONS: This result further supports the idea that SSPs, and especially G872, are of potential interest for treatment of recurrent stone disease.

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Analysis of VarianceAnimalsCalcium OxalateCells, CulturedCrystallizationDogsKidneyPolysaccharidesSurface PropertiesJournal ArticleResearch Support, Non-U.S. Gov't

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C F Verkoelen, J C Romijn, L C Cao, E R Boevé, W C De Bruijn, F H Schröder. 1996. Crystal-cell interaction inhibition by polysaccharides.. https://pubmed.ncbi.nlm.nih.gov/8558718/

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