RNA tertiary structure mediation by adenosine platforms.

J H Cate; A R Gooding; E Podell; K Zhou; B L Golden; A A Szewczak; C E Kundrot; T R Cech; J A Doudna

doi:10.1126/science.273.5282.1696

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PubMed · 8781229

RNA tertiary structure mediation by adenosine platforms.

J H Cate·A R Gooding·E Podell·K Zhou·B L Golden·A A Szewczak·C E Kundrot·T R Cech·J A Doudna

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Abstract

The crystal structure of a group I intron domain reveals an unexpected motif that mediates both intra- and intermolecular interactions. At three separate locations in the 160-nucleotide domain, adjacent adenosines in the sequence lie side-by-side and form a pseudo-base pair within a helix. This adenosine platform opens the minor groove for base stacking or base pairing with nucleotides from a noncontiguous RNA strand. The platform motif has a distinctive chemical modification signature that may enable its detection in other structured RNAs. The ability of this motif to facilitate higher order folding provides one explanation for the abundance of adenosine residues in internal loops of many RNAs.

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Adenosine Animals Base Composition Hydrogen Bonding Introns Models, Molecular Nucleic Acid Conformation RNA, Catalytic RNA, Protozoan Tetrahymena thermophila Comment Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J H Cate, A R Gooding, E Podell, K Zhou, B L Golden, A A Szewczak, C E Kundrot, T R Cech, J A Doudna. 1996-09-20. RNA tertiary structure mediation by adenosine platforms.. https://doi.org/10.1126/science.273.5282.1696

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