Rapid DNA quantification method in microplates using daunorubicine fluorescence quenching.

M Gagne; M Page

doi:10.3892/or.5.3.653

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Rapid DNA quantification method in microplates using daunorubicine fluorescence quenching.

M Gagne·M Page

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Abstract

We have developed a rapid, sensitive and inexpensive method to quantify DNA in crude extracts or PCR reaction products using daunorubicine fluorescence quenching. We obtained a linear standard curve from 4.7 ng to 600 ng DNA and no interaction was observed in the presence of proteins.

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M Gagne, M Page. Rapid DNA quantification method in microplates using daunorubicine fluorescence quenching.. https://doi.org/10.3892/or.5.3.653

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