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At least 1,387 records · Page 77Linked to original sources

PubMed1984

A software package for the calculation and statistical analysis of 51Cr-release assays.

A software package has been developed to calculate, store, and statistically analyze the results of 51Cr-release assays. It is written in Applesoft BASIC, is 'user friendly', and contains error trapping routines to catch most common mistakes. The greeting program contains a master menu from which one of the following programs may be called up: (1) 51Cr-release calculation, (2) statistical analysis, or (3) file manager. These programs give the user the capability to rapidly reduce cpm data to % specific release and statistically analyze the results using randomized design analysis of variance (ANOVA) and Turkey's honestly significant difference (HSD) test.

Chromium Radioisotopes↗

PubMed1987

Software for the quantitative evaluation of in vitro monoclonal antibody production from ELISA data.

Software has been developed which permits the quantitation of monoclonal antibodies secreted by B cell hybridomas. This program does not require the user to enter a large number of complex parameters and can be easily used without any previous computer experience. It fits all the experimental and standard curves by determining overlapping linear domains using the linear least-squares method. The program is based on logarithmic interpolations for determining Ig concentrations comparing experimental samples to Ig concentrations in standards. It provides a complete print-out of the data with editing options and is written in BASIC EDEX 4.0 Commodore computer language. It permits the accurate quantification of minute amounts of monoclonal antibodies and can be used to detect the inhibitory or enhancing effects of lymphokines or cytokines on Ig secretion by hybridoma B cells.

Antibodies, Monoclonal↗

PubMed1985

Software simulation of the EEG.

The literature contains many examples of digital procedures for the analytical treatment of electroencephalograms, but there is as yet no standard by which those techniques may be judged or compared. This paper proposes one method of generating an EEG, based on a computer program for Zetterberg's simulation. It is assumed that the statistical properties of an EEG may be represented by stationary processes having rational transfer functions and achieved by a system of software filters and random number generators. The model represents neither the neurological mechanism response for generating the EEG, nor any particular type of EEG record; transient phenomena such as spikes, sharp waves and alpha bursts also are excluded. The basis of the program is a valid 'partial' statistical description of the EEG; that description is then used to produce a digital representation of a signal which, if plotted sequentially, might or might not by chance resemble an EEG, that is unimportant. What is important is that the statistical properties of the series remain those of a real EEG; it is in this sense that the output is a simulation of the EEG. There is considerable flexibility in the form of the output, i.e. its alpha, beta and delta content, which may be selected by the user, the same selected parameters always producing the same statistical output. The filtered outputs from the random number sequences may be scaled to provide realistic power distributions in the accepted EEG frequency bands and then summed to create a digital output signal, the 'stationary EEG'.(ABSTRACT TRUNCATED AT 250 WORDS)

Biomedical Engineering↗

PubMed1992

PUMPSIM: a software package for simulating computer-controlled drug infusion pumps.

Advances in microcomputer technology have lead to the development of volumetric infusion pumps which can be controlled by a remote computer. Such pumps offer powerful capabilities for the administration of medications and fluids. Complex control strategies can be preprogrammed and the pump allowed to run with a minimum of supervision. The safety of patients is of the utmost importance. Therefore, great care must be taken to implement robust error avoidance, detection and correction protocols in the host control program. This paper describes a software simulator of a commercial infusion pump (Abbott Lifecare Model 4). Using the simulator in the development and debugging of control programs has several advantages over using the real pump: it provides detailed pump status information and it can stimulate various error and alarm conditions to comprehensively test the error recovery procedures of the control program.

Computer Simulation↗

PubMed1985

Software for electrophysiological experiments with a personal computer.

These programs were written for the IBM personal computer equipped with a Tecmar Labmaster analog interface board. The software operates under the MS-DOS operating system; it is written in compiled BASIC and employs short machine-language subroutines for crucial functions. Details are presented on the analog interface routines which make special use of the 9513 counter/timer chip on the Labmaster. Time resolution is about 32 microseconds per sample on the PC and 15 microseconds on the PC/AT. One series of programs performs traditional voltage- and current-clamp experiments on macroscopic currents in whole cells. A second series interfaces with a hardware window discriminator to capture single-channel events in a recirculating buffer; a stimulus can be triggered to fire during an open channel. Off-line programs perform standard analyses, and further processing can be performed with standard spreadsheet programs.

Animals↗

PubMed1988

A low-priced computer-assisted densitometer and analysis software for quantitative autoradiography.

A computer-assisted densitometer which consists of a darkroom enlarger, a black-and-white exposure meter, an amplifier, an analog-to-digital converter, and a microcomputer with a monitor and a graphic printer can be utilized for the quantitative analysis of autoradiograms. QUANTAR, a computer program written in BASIC, records the density measurements and stores the data in a file that can be easily retrieved by commercially available spreadsheet software. A spreadsheet template was designed to convert the digital readings into concentration of ligand in tissue. A variety of spreadsheet templates can be created to analyze data for a standard curve, a Scatchard plot, or a binding competition curve. This system, which is easy to assemble, may be useful to the frugal investigator with a modest equipment budget.

Adenosine↗

PubMed1989

Enhancing high-speed digitization of single-unit neuronal activity on a microcomputer using a hybrid software-hardware technique.

A new data acquisition technique allows a microcomputer simultaneously to digitize spikes at high rates, analyze spike waveforms for computer-based spike separation and manage other control tasks. The technique has two key features: a software scheduling routine written in a high-level language and a hardware analog delay of neuronal signals using simple hardware external to the computer. The technique provides an alternative for real-time data acquisition and can be used on microcomputers without requiring interrupt processing and assembly language programming.

Action Potentials↗

PubMed1991

Software filter for detecting spikes superimposed on a fluctuating baseline.

We describe and evaluate a software procedure for detecting and discriminating action potentials superimposed on a fluctuating DC baseline. Using an algorithm implemented in Fortran and Assembly language, spikes are detected by comparing the low-pass filtered first derivative of the signal with a preset threshold constant. This detection algorithm efficiently removes the DC baseline fluctuations usually encountered in sensory receptor recordings. It can function in real-time depending on the specific hardware configuration and program implementation. This approach enables the user to acquire direct DC-amplified signals, minimizing filter distortions of the action potential waveform.

Action Potentials↗

PubMed1992

Application of compiled BASIC in developing software for collection and analysis of neuronal firing frequency data.

Two programs are described which use an IBM-AT compatible personal computer, equipped with an analog-to-digital converter, to collect and analyze electrophysiological data. The first program is used to determine neuron firing rates during intracellular experiments and to save these results on disk. The second program is used to perform off-line analysis of the frequency data. Both programs are written entirely in the well known BASIC language and the Microsoft QuickBASIC compiler is employed for their use. All of the necessary hardware can be purchased commercially. In this paper emphasis is placed on the strategies and limitations involved when this high-level language is applied to tasks often needed in data acquisition and analysis. Both on- and off-line collection schemes are considered. This software is available from the authors.

Animals↗

PubMed1995

Design of the EPC-9, a computer-controlled patch-clamp amplifier. 2. Software.

The EPC-9 computer-controlled amplifier has no front-panel controls; therefore the user interface to the EPC-9 patch-clamp amplifier is defined entirely by software. This paper describes various user interfaces that have been implemented, including a high-level programming interface, a user interface based on the PostScript language, and graphical user interfaces that control the EPC-9 from data-acquisition programs. Also described are the algorithms used for automatic adjustment of the C-Fast and C-Slow transient cancellation circuitry. An overview of the procedures that perform automatic testing and calibration is given.

Animals↗

PubMed1994

JPCalc, a software package for calculating liquid junction potential corrections in patch-clamp, intracellular, epithelial and bilayer measurements and for correcting junction potential measurements.

The JPCalc program has been designed to graphically illustrate how junction potential contributions arise in various electrophysiological situations, to enable the magnitude and direction of those values to be readily calculated and to show clearly how the resultant appropriate corrections need to be applied to experimental measurements. In particular, the program covers the analysis of junction potential contributions and corrections in all the various different patch-clamp configurations (intact patches, inside-out patches, outside-out patches, whole-cell recording and perforated patches), intracellular recording, epithelial and bilayer measurements. It can also be used to correct experimental measurements designed to try to directly measure junction potentials and to compare both the magnitude and direction of such corrected measurements with their theoretical values. Although it is recommended that all of the above measurements be done with salt-filled electrodes or salt bridges, the program will allow the use of Ag/AgCl reference electrodes in direct contact with bathing solution in most situations. JPCalc also makes use of a library file of mobility and valency data for about 30 ions which can be extended by the user. In addition to its value in correcting electrical measurements, the program with its graphical displays may also be of value in focusing discussion about the contribution of junction potentials to such measurements and in helping to design experiments to either minimize their contribution or to measure their values. The JPCalc program, designed for IBM-compatible computers with color or monochrome monitors, can also be run on Macintosh computers with SoftPC emulation software.

Animals↗

PubMed1994

Semiquantitative analysis of in-situ hybridization results using IMAGE software: a rapid method for counting reduced silver grains over mRNA-positive cells.

The advent of microcomputers has brought about a revolution in the computing power available to the average user. Image analysis is a very resource-intensive process, making great demands on computing power, memory, and display capabilities of most computers. Thus, in the past, dedicated, single-use hardware and software had to be custom made for environments requiring image analysis. We present here an easy-to-use image analysis protocol available to most users with a Macintosh II series computer and access to IMAGE (a public domain image analysis program). The protocol allows for semi-quantitation of silver grains over cells used in the interpretation of in-situ hybridization results. We show that the method provides a quick and reliable means of counting grains over mRNA-positive cells in an automated fashion. We also provide evidence that the method can be used to detect differences between experimental treatments.

Animals↗

PubMedDate not supplied

A Windows software package to record from voltage-clamped Xenopus oocytes.

We have written a software package to record, display and analyze membrane currents elicited by neurotransmitter receptors or voltage-activated channels in voltage-clamped Xenopus oocytes. This suite, which consists of 4 applications, runs on IBM-PC compatible microcomputers under Windows 3.1. The recording programs use Direct Memory Access (DMA) to access the analog-digital board. The first program, NicPulse, is aimed at studying voltage-activated channels. It delivers voltage steps to the voltage-clamp and records the resulting membrane current. The second program, NicScope, emulates a dual-trace digital oscilloscope. It operates either in continuous or triggered mode, and is used chiefly to display neurotransmitter-induced responses in oocytes. The third recording program, VRamp, automatically determines the voltage-current relationship of drug-activated responses (I/V curve), by applying a voltage ramp and recording the subsequent clamping current. The last program, NicView, is designed to analyze records taken with NicScope and NicPulse. The present paper will discuss several issues regarding the design of these programs, and will give a brief description of each application.

Animals↗

PubMed1985

Health and medical software related to computer user satisfaction: an interactive online expert system using diagnostic audit trails through telecommunication networks.

The selection of a health and medical software (HMS) system is a complicated process. The overall satisfaction derived from a system depends on many variables. This study analyzes the influence of HMS predictor variables on overall satisfaction as determined by multiple regression. The null hypothesis, that multiple correlation coefficient is zero, is rejected. Th alternative hypothesis is accepted. This study confirms the theories that suggest that HMS ease of operation, independent variables, technical support troubleshooting, and reliability of computer are the major determinations of overall computer user satisfaction. The factors identified were used in the design of an HMS expert system based on artificial intelligence (ESAI).

Computers↗

PubMed1986

Software for analysis of equine ground reaction force data.

Software for analysis of force plate recordings of the horse at normal walk is described. The data of a number of stance phases are averaged to obtain a representative tracing of that horse. The amplitudes of a number of characteristic peaks in the force-time curves are used to compare left and right front limbs and left and right hind limbs. The averaged tracings are plotted, default on the line printer or, via separate program, on a high quality pen plotter. A version of the program applicable for analysis of human force plate recordings, is available.

Animals↗

PubMed1987

PRONUC: a software package for the analysis of protein and nucleic acid sequences.

PRONUC is a menu-driven software package from which a molecular biologist may gain access to a variety of tools for the analysis of protein and nucleic acid sequences. Features include various algorithms for sequence comparisons, secondary structure prediction, sequence manipulation (translation complementation etc.) and finding restriction enzyme cut-sites. The sequences under study can be retrieved from several databases of published sequences or a users sequence(s) can be entered by means of a sequence editor or retrieved from a database constructed by the user. PRONUC comes with a comprehensive manual and on-line help which reflects several years of user feedback and is available for Digital VAX computer systems running the VMS or micro-VMS operating system.

Algorithms↗

PubMedDate not supplied

Versatile software for collection, analysis and plotting of biochemical data using the IBM PC.

A software package, OMNILAB, has been written for the IBM PC, XT and AT computers to be a general purpose system for data collection, analysis, and display. The program supports collection of dta from a variety of absorbance detectors, from pH meters and from other instruments that output time-varying analog voltages in the ranges of millivolts or volts. The program includes capabilities for averaging of data, baseline subtraction, integration of curves, and versatile formatting for both video and hardcopy display of data.

Data Collection↗

PubMed1989

Data processing software for electrophysiological visual exploration.

The software we have developed originated after careful study of a routine functional visual processing session. It has three objectives: (i) to have available a large library of data processing programs; (ii) to add to these electrophysiological results, automatically transferred into a file, a clinical record card, creating a personalized file for study of visual characteristics of each patient; (iii) to allow programs providing statistical studies, creation of groups based on the same clinical features and various comparisons between files or groups. The database now contains 5000 different files and the results of its use are very encouraging. Some results are presented, especially those of a study on multiple sclerosis. The goal of the operation is the creation, in the future, of an expert system.

Clinical Laboratory Information Systems↗