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Re-fixation in a lymph node revealing solution is a powerful method for identifying lymph nodes in colorectal resection specimens.

A Svec·L Horák·J Novotný·P Lysy
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Abstract

AIM: To verify an impact of a lymph node revealing solution (LRS) on the number of lymph nodes (LN) revealed in colorectal resection specimens. MATERIAL AND METHOD: Fifty-nine cases of rectosigmoid carcinomas divided into two subgroups-stage pT2&3 (20 cases) and ypT2&3 (with pre-operative radiotherapy-39 cases)-were formalin fixed for 42-72 h, serially sectioned and re-fixed in LRS containing ethanol, diethyl ether, glacial acetic acid, and formalin for 24 h. The number of LNs was matched to a control group consisting of 34 cases of colorectal carcinoma, 14 pT2&3 and 20 ypT2&3 rectosigmoid carcinomas examined routinely. Results were statistically tested by Student's t-test. RESULTS: In the study group the total number of revealed LNs was 869, range 1-48, average 14.7 per specimen, median 15. In the control group the number of LNs was 200, range 0-13, average 5.9 per specimen, median 6. The difference between the study and the control group was statistically significant (P = 0.05). CONCLUSION: Using LRS leads to a significant increase in the number of revealed LNs in colorectal resection specimens and makes it possible to find more than 12 LNs in a majority of stage pT2 and pT3 adenocarcinomas.

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Acetic AcidAdenocarcinomaColectomyColorectal NeoplasmsDiagnosis, DifferentialEthanolEtherFormaldehydeHumansIn Vitro TechniquesLymph Node ExcisionLymph NodesLymphatic MetastasisReproducibility of ResultsRetrospective StudiesTissue FixationComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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A Svec, L Horák, J Novotný, P Lysy. 2006-03-13. Re-fixation in a lymph node revealing solution is a powerful method for identifying lymph nodes in colorectal resection specimens.. https://doi.org/10.1016/j.ejso.2006.02.002

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