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Preparation of type 2 herpes simplex virus complement-fixing antigen.

E L Palmer·M L Martin·D T Warfield
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Abstract

Comparable complement-fixing antigens of type 1 and type 2 herpes simplex virus were produced by extraction of infected African green monkey cells with 0.85% NaCl which was buffered at pH 9.0 with 0.05 m glycine-NaOH. The optimal antigen dilutions were higher in titrations against hyperimmune animal sera than in titrations against human sera. Complement-fixing antibody to type 2 herpes antigen was detected in 5 of 17 sera from healthy humans.

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AnimalsAntibodiesAntigensAntigens, ViralCell LineComplement Fixation TestsCytopathogenic Effect, ViralDiagnosis, DifferentialGlycineHaplorhiniHerpes SimplexHumansHydrogen-Ion ConcentrationImmune SeraImmunochemistryKidneyRabbitsSerotypingSimplexvirusSodium ChlorideSodium HydroxideUltrasonicsVibrationJournal Article

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E L Palmer, M L Martin, D T Warfield. 1971. Preparation of type 2 herpes simplex virus complement-fixing antigen.. https://doi.org/10.1128/am.22.5.925-927.1971

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