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Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry.

M Wigger·J P Nawrocki·C H Watson·J R Eyler·S A Benner
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Abstract

A model experiment for the 'on-line' screening of substrate libraries by enzymes using combinatorial libraries in combination with electrospray ionization-Fourier transform ion cyclotron resonance (ESI-FTICR) mass spectrometry has been performed. The reaction between the electrophilic substrate 1-chloro-2,4-dinitrobenzene and component of a H-gamma-Glu-Cys-Xxx-OH library, catalyzed by glutathione-S-transferase, has been monitored. It shows the feasibility of 'two-dimensional' screening of substrate libraries by ESI-FTICR mass spectrometry.

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CyclotronsDinitrochlorobenzeneFourier AnalysisGlutathione TransferaseLibrariesMass SpectrometrySubstrate SpecificityJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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BibTeXRIS

M Wigger, J P Nawrocki, C H Watson, J R Eyler, S A Benner. 1997. Assessing enzyme substrate specificity using combinatorial libraries and electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometry.. https://doi.org/10.1002/(sici)1097-0231(19971030)11%3A16%3C1749%3A%3Aaid-rcm91%3E3.0.co%3B2-g

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