[A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen].

V A Kutuzov; V V Mikhaĭlov; S E Kruglova; V V Kutuzov; A P Kirillov; V P Krasnianskiĭ; V A Pshenichnov; E V Lebedinskaia

PubMed · 8073738

[A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen].

V A Kutuzov·V V Mikhaĭlov·S E Kruglova·V V Kutuzov·A P Kirillov·V P Krasnianskiĭ·V A Pshenichnov·E V Lebedinskaia

Abstract

A comparative evaluation of the developed variants of indirect solid-phase enzyme immunoassay using fluorogenic and chromogenic substrates for the determination of Marburg virus antigen was carried out. The resolving capacity of this method was 3.8 x 10(-9) g of protein for the former and 3.1 x 10(-8) g of protein for the latter substrate. Cross titrations demonstrated the lack of common antigens with Ebola virus.

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Animals Antigens, Viral Ebolavirus Evaluation Studies as Topic Hymecromone Immunoenzyme Techniques Indicators and Reagents Marburgvirus Nitrophenols Organophosphorus Compounds Rabbits Comparative Study English Abstract Journal Article

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V A Kutuzov, V V Mikhaĭlov, S E Kruglova, V V Kutuzov, A P Kirillov, V P Krasnianskiĭ, V A Pshenichnov, E V Lebedinskaia. [A comparative evaluation of an immunoenzyme analytical method using a fluorogenic or chromogenic substrate in determining the Marburg virus antigen].. https://pubmed.ncbi.nlm.nih.gov/8073738/

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