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PubMed · 9011156

Immunoreactive cytokines within primates.

W Ahne·A Mayr·H Wiesner
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Abstract

Peripheral blood mononuclear cells of primates (man, orang utan, gorilla, baboon), rodents (mouse, rat), carnivores (cat, dog), artiodactyls (cattle, goat, pig) and perissodactyls (horse) were isolated and stimulated with mitogens (5 micrograms/ml LPS, 5 micrograms/ml PHA) at 37 degrees C. Cytokines immunoreactive to monoclonal antibodies (mAb) directed to human cytokines (TNF-alpha, IL-1 alpha, IL-2, IL-6, IFN-gamma) could be detected by enzyme-linked immunosorbent assay (ELISA) in the case of primates only. The mAb used did not recognize cytokines of the other mammalian species investigated. The results demonstrate the close relationship within the primates from the immunophysiological point of view.

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AnimalsAntibodies, MonoclonalCatsCattleCells, CulturedCytokinesDogsEnzyme-Linked Immunosorbent AssayGoatsGorilla gorillaHorsesHumansLeukocytesLipopolysaccharidesMiceMitogensPapioPhylogenyPhytohemagglutininsPongo pygmaeusPrimatesRatsSwineJournal Article

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BibTeXRIS

W Ahne, A Mayr, H Wiesner. 1996. Immunoreactive cytokines within primates.. https://doi.org/10.1111/j.1439-0450.1996.tb00359.x

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